Comparative Omics of extracellular microvesicles isolated using Vn* affinity peptides

Details: Conference Paper

We have engineered and validated a synthetic peptide (Vn96) with specific affinity for canonical heat shock proteins (HSPs) as a tool for rapid isolation and purification of HSPcontaining extracellular microvesicles (eMVs). In most cancers HSP expression is exceptionally high (chaperonopathies) and found on the surface of their secreted eMVs. Thus, our method isolates cancer specific eMVs of highest clinical value and also compares favourably in terms of efficiency, cost and platform versatility. As part of the validation process for Vn peptides, we performed comparative genomic and proteomic characterizations of the eMV subsets obtained by Vn affinity protocol with those from established protocols such as ultracentrifugation and a commercial isolation reagent. The compatibility of Vn affinity technology with such downstream "omics" applications as next-generation sequencing (NGS) and mass spectrometry (MS) is essential for its ultimate use in the search for clinical biomarkers present in eMVs.
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