Novel Peptide with affinity for canonical heat shock proteins (HSPs) as a tool for capture and enrichment of extracellular microvesicles

Details: Conference Paper

Here we describe the development and validation of a peptide (Vn96*) with specific affinity for heat shock proteins (HSPs) and their complexes as tools for isolation and purification of HSP containing extracellular
Microvesicles (eMVs) and/or Exosomes (together referred as eMVs here) from cell culture media, human and animal body-fluids. We compare the specificity of the Vn96 peptide for the isolatation of eMVs from different sources with conventional exosome preparation by ultracentrifugation and a commercially available Kit.
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